| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4addb5bc1c280e23fda6941011511797
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49790ed5b8ddc5fa27a1d47732c612df |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-01002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate |
2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b648908fdb5a3f8aa48c92d287713079
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fdb27c73166e4c2c24e1702aab56444
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5404482c73d3f5e3d720d035bbef75f5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ece519c57098eb72097d663494ef3fe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54679fac02c9a672d667657ae402d8de
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74eb02304fcc9441973c361625059fc4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4adb942615f40a203b240823b5ed8ba5 |
| publicationDate |
2020-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-111434770-A |
| titleOfInvention |
Expression and self-processing of protein glutaminase proenzyme in Bacillus subtilis |
| abstract |
The invention discloses a method for producing protein glutaminase by using Bacillus subtilis, and belongs to the technical field of biological engineering. The method of the invention carries out codon optimization for Bacillus subtilis to obtain a protein glutaminase precursor (pro-PG) gene fragment suitable for expression in the Bacillus subtilis expression system, and connects the gene fragment with the expression vector pHT43 to construct a The recombinant vector pHT43-pp is transformed into Bacillus subtilis to form recombinant Bacillus subtilis, and the fermentation is induced by inducers such as lactose. Mature protein glutaminase or mPG. The invention includes the following advantages: a method for expressing pro-PG by using Bacillus subtilis is developed; a method for self-processing pro-PG by using a Bacillus subtilis expression system is developed, without the addition of exogenous protease, which can be efficient, fast and efficient. It produces mPG in a directional manner, and has high enzymatic activity, which is suitable for industrial production. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114015678-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111394331-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111394331-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4242303-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023170177-A1 |
| priorityDate |
2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |