| abstract |
The invention discloses a glutamine transaminase and its encoding gene, an expression vector and a recombinant bacteria, and belongs to the technical field of genetic engineering. The present invention carries out genetic modification through codon optimization technology, takes Pichia pastoris as a host, selects a secretory expression vector, transforms it into a transglutaminase gene whose homology with the Bacillus subtilis source gene is 70.6% after transformation, and directly secretes and expresses it. The target protein was obtained with a high copy number of recombinant engineering bacteria. The optimal pH preference of the recombinant engineering bacteria shifted to acidity, from pH 7 to 6, and the relative enzyme activity increased from 71.4% to 89.7% after a water bath at 50 °C for 30 min. This promotes the future industrial production and wide application of transglutaminase in various fields. |