| abstract |
The invention discloses a disperse Pantoea for producing trehalase, and separation, screening and application thereof, wherein the trehalase producing strain C3 is identified as the disperse Pantoea dispersa, is preserved in the China general microbiological culture Collection center, has the preservation time of 2019, 3 and 25 days and has the preservation number of CGMCC No. 17445. The strain C3 is obtained by screening a soil sample by using trehalose as a unique carbon-nitrogen source through flat plate primary screening, shaking flask secondary screening, enzyme activity determination and trehalase activity as an index. The Pantoea dispersa C3 can effectively produce trehalase in large quantity, the trehalase has obvious advantages in temperature and pH stability, can be compounded with other enzymes to be applied to the ethanol industry, can improve the utilization rate of raw materials of the ethanol industry, and has wide application prospects in the ethanol industry. |