| abstract |
The present invention provides a pyrrole-merocyanine derivative fluorescent probe and a preparation method and application thereof, wherein the chemical structural formula of the pyrrole-merocyanine derivative is as follows: The preparation method is: N-morpholinoethyl-2,4-dimethyl-5-formylpyrrole-3-formamide and N-ethylbenzothiazole iodonium salt are dissolved in an organic solvent; the obtained solution is dissolved Piperidine was added dropwise as a catalyst, and then the reaction was stirred under reflux for 3-4 h at 80 °C; then cooled to room temperature, filtered under reduced pressure, the obtained solid residue was washed with ethanol, and then recrystallized with ethanol to obtain the pyrrole-merocyanine Derivative fluorescent probes. The fluorescent probe of the pyrrole-merocyanine derivative of the present invention can selectively interact with hypochlorite under the physiological conditions of pure water phase, the solution yellow fades, and the green fluorescence is significantly weakened, especially as a fluorescent probe in cell lysozyme Application of convenient detection of hypochlorite in vivo. |