http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110767262-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B20-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B20-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B5-20 |
| filingDate | 2019-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110767262-B |
| titleOfInvention | Structure-based optimized design method for aptamer |
| abstract | The invention belongs to the technical field of nucleic acid aptamer design, and particularly relates to a structure-based nucleic acid aptamer optimal design method. The invention starts from the 3D structure of the aptamer obtained by SELEX technology screening, and obtains the 3D structure of a compound formed by small molecule combined aptamer through spontaneous combination simulation; analyzing the structure and key binding sites of the complex, and guiding the optimal design of the high-affinity aptamer by using the obtained information; further, the design scheme was evaluated using energy profiles of the surface binding energy of the aptamer. The invention has been used in the optimized design of the aptamer GO18-T-d which specifically binds to the toxin GTX 1/4; the aptamer was screened by SELEX technology with an affinity of 75.63 nM. A truncated aptamer (GO18-T-d _ S) of GO18-T-d is obtained through optimization, and the affinity of the aptamer is improved by 20 times through MST experiment verification; the optimization method is reasonable and effective. |
| priorityDate | 2019-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.