http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110396535-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f90c1df3857ee72900e0015c75caecd9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
| filingDate | 2019-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fc33566fa224db566989855df9792ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c11bed0892e70162165f65208319c75b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_407d9e280ff798fe11c855203a9c87de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_932b77d1eab3d782d1317421bb7d148e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53ef97c2fcec313bbdf0effc78b675a9 |
| publicationDate | 2019-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110396535-A |
| titleOfInvention | A method for detecting site-specific binding of CXXC domains regulated by methylation of CGI sequences by single-molecule technology |
| abstract | The invention provides a method for detecting site-specific binding of CXXC domains regulated by methylation of CGI sequences. By constructing a CGI hairpin structure and connecting the CGI hairpin structure to the bottom surface of the reaction pool and the surface of the microsphere, under the control of the single-molecule manipulation technology, the microsphere is subjected to tension, and then the CGI hairpin is released without the presence of the CXXC domain. The clip structure is stretched, and the CGI hairpin structure is quickly opened; when there is a CXXC domain, the opening process of the CGI hairpin structure is hindered by the CXXC domain bound to it, resulting in a pause signal; for unmodified Count the number of pause signal events in the CGI sequence and the methylation-modified CGI sequence, and judge the change of the binding of the CXXC domain to the specific site of the CGI sequence in different states of epigenetic modification, thereby revealing the epigenetics of CGI The effect mechanism of genetic modification on gene transcription activity provides a single-molecule level experimental detection method for understanding the life activities of cells and the mechanism of canceration of cells. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111254144-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111721504-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111721504-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111254144-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111269911-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111269911-A |
| priorityDate | 2019-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.