| abstract |
The invention relates to a method for determining the content of polymers in cephalosporin antibiotics. A method for determining the content of polymers in cephalosporin antibiotics, comprising the steps of: detecting the test solution and the control solution respectively according to high performance liquid chromatography, so as to obtain the chromatogram of the test solution and the chromatogram of the control solution Figure, wherein, need testing solution is the solution that contains cephalosporin antibiotic drug, and contrast solution is the diluent of need testing solution, and chromatographic condition is: chromatographic column is octadecylsilane bonded silica gel column, mobile phase includes mobile phase A and mobile phase B, mobile phase A comprises water, and mobile phase B comprises methanol; According to the chromatogram of need testing solution and the chromatogram of contrast solution, adopt the content of the polymkeric substance in the cephalosporin antibiotic drug of self-contrast method of principal component calculation . It has been proved by experiments that the determination of the content of the polymer in the above-mentioned cephalosporin antibiotics is relatively accurate. |