http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109932444-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
filingDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109932444-B |
titleOfInvention | A method for evaluating post-translational modifications of a glycoprotein with multiple charge variants |
abstract | The invention discloses a method for evaluating post-translational modification of multiple charge isomers of glycoproteins. The method includes the following steps: 1) separating a variety of charged heterogeneous bodies by isoelectric focusing electrophoresis; 2) cutting and collecting the bands after the separation of a plurality of charged heterogeneous bodies; 3) collecting a variety of heterogeneous bodies The plastids were subjected to in-gel digestion respectively; 4) The peptides after in-gel digestion were extracted; 5) The extracted peptides were analyzed by mass spectrometry to confirm post-translation such as oxidation, deamidation, C-terminal Lys deletion and glycosylation. retouch. The method of the invention overcomes the difficulty of analyzing post-translational modifications of multiple charge isomers, can accurately analyze multiple post-translational modifications of single-charge glycoproteins, and is more conducive to the analysis of multiple post-translational modifications related to multiple charge isomers. It is suitable for the analysis and evaluation of post-translational modifications of various charged heterosomes of glycoproteins. |
priorityDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.