http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109932444-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b946f8a160a77d9f664816a704364d83 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73fdaa6264874ef99d7d732ab9c283c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_943b60335f2ed28311afcf62ac8e1ff1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3049b19f27a6ea14be6fd7fe3d775e82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_506d06b44aeb4458f0919a2a66b986b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3be9119d53b9ccf76028fc9df81a8768 |
| publicationDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109932444-A |
| titleOfInvention | A method for evaluating post-translational modifications of a glycoprotein with multiple charge variants |
| abstract | The invention discloses a method for evaluating the post-translational modification of various charge isomers of glycoproteins. The method includes the following steps: 1) separating a variety of charged heterogenous bodies by isoelectric focusing electrophoresis; 2) cutting and collecting the bands after the separation of the multiple charged heterogenous bodies; 3) collecting a variety of heterogenous bodies The plastids were subjected to in-gel digestion respectively; 4) The peptides after in-gel digestion were extracted; 5) The extracted peptides were analyzed by mass spectrometry to confirm post-translation such as oxidation, deamidation, C-terminal Lys deletion and glycosylation. retouch. The method of the invention overcomes the difficulty of analyzing post-translational modifications of multiple charge isomers, can accurately analyze multiple post-translational modifications of single-charged glycoproteins, and is more conducive to the analysis of multiple post-translational modifications related to multiple charge isomers. It is suitable for the analysis and evaluation of post-translational modifications of various charged heterosomes of glycoproteins. |
| priorityDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 92.