http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109913421-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_008c9107ff464e201e724ad32b7630cc |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5de648685e81e59de395def7ecd3d032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea7a46e6af2b77d5b925447417154d9c |
| publicationDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109913421-A |
| titleOfInvention | Construction and identification of tumor cell lines with stable and high expression of midkine protein |
| abstract | The invention discloses a method for constructing and identifying a tumor cell line with stable and high expression of midkine protein, which is characterized in that: a DNA sequence encoding midkine is fused into an expression vector pCDNA3.1, and tumor cells are transfected, and the high expression of midkine is established through geneticin screening. For cell lines, use Western Blot to confirm that the expression level of midkine protein is increased; through the proliferation curve measurement, it is found that the proliferation rate of high-expressing midkine cells is significantly accelerated; the protein of normal and low-glucose cultured cells is extracted, and Western Blot experiments are performed to prove that cells with high midkine expression AMPK phosphorylation activation was inhibited in midkine, which was consistent with the functional characteristics of midkine protein. The above results proved that the midkine high-expressing cell line was successfully constructed, and it could positively regulate cell proliferation and inhibit the activity of AMPK pathway. The establishment of this method provides a method for the construction and identification of a stable and high-expressing cell line of midkine protein, and provides conditions for studying the function of midkine protein, revealing its mechanism of affecting cell proliferation and AMPK pathway, and providing a new idea for the clinical application of midkine. . |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112980859-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112986571-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112986571-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112980859-B |
| priorityDate | 2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 109.