| abstract |
The invention discloses an active small molecule probe targeting mitochondria, a preparation method and application thereof. The active probe contains three kinds of modifications: a mitochondrial positioning group, a reactive group and a micro-orthogonal group, the synthesis process is simple, and the application is more convenient. Efficient and fast. They can be precisely targeted and enriched in mitochondria, selectively reactively labeled with endogenous mitochondrial proteins, especially through the introduction of alkynyl groups, allowing probes to bind to biotin-streptavidin, thereby obtaining more much protein. The probe of the invention abandons the traditional and complicated mitochondrial separation steps, and extracts high-purity mitochondrial proteome by directly breaking the sample, so as to capture more mitochondrial proteins more effectively and conveniently, and further improve the "catalog" of mitochondrial proteomics. provide new means. |