| abstract |
The invention discloses a Bacillus B8W22 and an application. The application comprises the following steps: using the wet cells obtained by fermentation and culture of Bacillus B8W22 or the dry cells obtained by freeze-drying the wet cells as a catalyst, racemic (R , S)-indoline-2-ethyl formate as the substrate, using the phosphate buffer with pH value of 7.0 to 8.5 as the reaction medium, at 25 to 40 ° C, 150-200rpm The preparation is carried out (S) -Indoline-2-carboxylic acid. The enzyme-producing microbial strain-Bacillus B8W22 provided by the invention has strong stereoselectivity, the product enantiomeric excess value is ≧93.7%, the conversion rate reaches more than 45.4%, the energy consumption is low, the environmental pollution is small, and is suitable for industrial production. |