http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109337892-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43586 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0059 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y110-03001 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-02 |
| filingDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109337892-B |
| titleOfInvention | Method for immobilizing taro polyphenol oxidase by using fibroin |
| abstract | The invention discloses a method for fixing taro polyphenol oxidase by fibroin, which comprises the following steps: the method comprises the steps of immobilizing the taro polyphenol oxidase by taking fibroin as a carrier, investigating immobilization temperature, immobilization time, pH value and solid-liquid ratio of PPO crude enzyme liquid to immobilized enzyme as adsorption conditions, detecting by an ultraviolet-visible spectrophotometry according to a Coomassie brilliant blue method, and determining the loading capacity, immobilized enzyme activity, activity recovery rate and enzyme adsorption content of the polyphenol oxidase in the immobilization process by using a standard curve and a calculation formula. The method has the advantages of low cost, simple operation and wide and easily-obtained raw materials, and the prepared immobilized enzyme has the stability of enzyme, keeps the high efficiency and specificity of the taro polyphenol oxidase, and has the characteristics of convenient separation and recovery, repeated use, good continuous operation controllability and the like. |
| priorityDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.