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filingDate 2018-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_581c528b0054f1c914706ae417a42f14
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publicationDate 2018-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108753801-A
titleOfInvention It can inhibit the soybean mosaic virus sequence and its application that Agrobacterium grows
abstract The invention discloses the soybean mosaic virus sequences that Agrobacterium can be inhibited to grow, and demonstrate the sequence and can be used in the screening for being directly realized by recombinant clone in Agrobacterium.Agrobacterium is the important host strain of plant conversion system, but the recombinant clone built needs to be transferred to Agrobacterium after screening in Escherichia coli.There is the characteristic for inhibiting Agrobacterium growth present invention finds Strains of Soybean Mosaic Virus SC15, and strain SC3 and SC7 do not have same effect;It is truncated and is tested by virus sequence, the present invention identifies the relevant sequence SC15P of Agrobacterium growth inhibition in SC15;By using the sequence construct and gateway cloning systems compatible vector and carry out that LR reacts and transformation experiment, the final present invention realize the imagination for carrying out recombinant clone directly in Agrobacterium using SC15P.
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