http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108535236-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-65 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-65 |
| filingDate | 2018-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108535236-B |
| titleOfInvention | Method for ultrasensitively detecting miRNA based on dual-amplification SERS signal system |
| abstract | The invention discloses an ultra-sensitive miRNA detection method based on a double-amplification SERS signal system. According to the invention, a layer of polydopamine is wrapped on the surface of a 4-MBA-marked gold nanoparticle to form an SERS mark with a gold-PDA-silver satellite structure, and a Raman signal of the SERS mark is greatly amplified by utilizing the unique local surface plasma resonance of gaps between gold and silver nanoparticles; meanwhile, the invention further amplifies the SERS signal by combining enzyme digestion cycle reaction with a magnetic capture mechanism, thereby realizing rapid high-sensitivity quantitative detection of miRNA. The method is simple to operate, good in detection stability and high in detection sensitivity; during operation, only the incubation liquid after enzyme digestion circulating reaction is sequentially added into the functionalized magnetic nanoparticles for reaction, and then reacts with the SERS marker, and detection can be immediately carried out through magnetic separation after the reaction is finished, so that rapid quantitative detection is realized; can be applied to the clinical diagnosis and screening of early cancer. |
| priorityDate | 2018-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.