http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107460178-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01078 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M47-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2494 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-12 |
| filingDate | 2017-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107460178-B |
| titleOfInvention | Preparation method of mannase and extraction refining device thereof |
| abstract | The invention discloses a preparation method of mannase and an extraction refining device thereof, which comprises the following steps of firstly obtaining fermentation liquor by liquid fermentation of pichia pastoris engineering strains, and then carrying out extraction refining: 1) circularly filtering and concentrating the fermentation liquor by adopting a ceramic membrane, and controlling the temperature of a circulating feed liquid to be below 20 ℃; 2) concentrating for a period of time, and circularly concentrating the filtrate obtained by filtering with ceramic membrane by ultrafiltration membrane equipment, wherein the temperature of the circulating feed liquid is controlled below 20 ℃; 3) and 3) when the volume of the concentrated solution in the step 2) is reduced to 1/5-1/10 of the volume of the filtrate, adding starch, uniformly mixing, and finally carrying out spray granulation to obtain the mannase. The ceramic membrane filtration is adopted, so that the production bacteria are intercepted, the filtration and sterilization effects are realized, the coupling with the ultrafiltration membrane concentration is realized, the process time is shortened, the proliferation risk of microorganisms is reduced, the qualified rate of the obtained finished product hygiene index is greatly improved to 99%, and the product yield is improved to more than 83%. |
| priorityDate | 2017-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 203.