http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105325289-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2014-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105325289-B |
| titleOfInvention | Overcome the vitrified method of carnation Shoot Tip Culture using hydroscopic high-molecular resin |
| abstract | The invention belongs to plant regeneration field, there is provided one kind overcomes the vitrified method of carnation Shoot Tip Culture, including step using hydroscopic high-molecular resin：1) the healthy and strong Carnation Flower of growth selection, cut and stay the 2cm stem sections with top；2) aseptic water washing 35 times, it is standby；3) strip carnation stem apex 0.5mm to be linked into culture medium, the culture medium is：Molysite doubles MS+ sucrose 30g/L+ agar 6g/L+TDZ1g/L+NAA1g/L+8g/L macromolecule water uptake resins, is cultivated afterwards at 20 30 DEG C.The present invention determines the selection of medium additives kind through overtesting, compares through overtesting, have selected the concentration of additive.Learnt by result of the test, the macromolecule water uptake resin of suitable concentration can be reduced to 17% or so to the vitrifying incidence after carnation red cultivar stem apex detoxification, and subsequently not vitrified plant occurs vitrified situation and is greatly reduced again. |
| priorityDate | 2014-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.