http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105300783-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 |
| filingDate | 2014-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105300783-B |
| titleOfInvention | A kind of method that glycopeptide segment solid phase is enriched with simultaneously mass spectral analysis |
| abstract | The invention belongs to protein analysis field, it is related to a kind of new method of magnetic Nano material enrichment glycosylated peptide using aminooxy group modification comprising: periodic acid is carried out to the hydroxyl on glycopeptide first and receives NaIO 4 Oxidation is so that hydroxyl becomes aldehyde radical, then the magnetic Nano material Fe that aminooxy group is modified 3 O 4 @ONH 2 In glycopeptide solution after being placed in oxidation, it is reacted between the aldehyde radical obtained after being aoxidized by sugar chain in aminooxy group group and glycopeptide, glycopeptide is fixed on magnetic Nano material, it will then not clean and remove with the non-glycopeptide of nano material reaction, finally recycling goes sugar chain enzyme to disintegrate down the glycopeptide of capture from material, is sent into mass spectral analysis glycosylated peptide.The method of the present invention step is simple and convenient to operate, rapidly and efficiently, and highly sensitive, the highly selective mass spectral analysis of glycosylated peptide may be implemented. |
| priorityDate | 2014-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 201.