abstract |
A method for the enzymatic regeneration of the redox cofactors NAD + /NADH and NADP + /NADPH in a one-pot reaction is disclosed. In this method, one of the two redox cofactors is obtained in its reduced form and the other Obtained in its oxidized form. The disclosed method is characterized in that: a) in the regeneration reaction which converts the reduced cofactor back to its original oxidized form, oxygen or a compound of formula R 1 C(O)COOH is reduced, and b) in the In the regeneration reaction in which the oxidized cofactor is converted to its original reduced form, the compound with the general formula R 2 CH(OH)R 3 is oxidized, and R 1 , R 2 and R 3 in the compound have different meanings. |