http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103601796-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 |
| filingDate | 2013-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103601796-B |
| titleOfInvention | The Petiolus Trachycarpiization method of modifying of Huntington protein |
| abstract | The invention discloses the Petiolus Trachycarpiization method of modifying of a kind of Huntington protein, comprise the following steps: (1), acquisition recombiant protein Htt(1 90): a, the acquisition of Huntingtin gene；B, recombinant expression plasmid pTWIN1 htt Structure；C, express the structure of recombinant escherichia coli strain DH5 α htt of Huntington protein；D, the expression of recombiant protein；(2), recombiant protein Htt(1 90) purification；(3), SPPS method obtains the Htt(Cys K92 K158 that Petiolus Trachycarpiization is modified) polypeptide；(4) Htt(Cys K92 K158) purification of polypeptide；(5) Htt(Cys K92 K158) polypeptide and recombiant protein Htt(1 90) obtain through coupling and to modify destination protein Htt(1 158 through Petiolus Trachycarpiization)；(6) through Petiolus Trachycarpiization modify destination protein Htt(1 158) purification.By the Petiolus Trachycarpiization of Huntington protein is modified, demonstrate that the stability of albumen increases, Petiolus Trachycarpiization albumen has the ability of antagonism hydrolytic enzyme, demonstrates that Petiolus Trachycarpiization albumen has stronger stability, and the Petiolus Trachycarpiization therefore studying Huntington protein is significant to the treatment of Huntingdon disease. |
| priorityDate | 2013-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.