| abstract |
The invention provides a novel surface chemically modified magnetic material and a method for enriching azide-marked biomacromolecules using the material. The invention combines the magnetic nanometer material with the separation strategy in the prior art, fixes the terminal alkynyl group on the superparamagnetic magnetic beads through a cleavable group, and forms a novel surface-modified magnetic bead containing the terminal alkynyl group. In addition, through Cu(I) catalyzed click chemical reaction, the azide group forms a covalent bond with the alkyne group on the surface of the magnetic bead; the superparamagnetism of the magnetic bead is used to enrich and separate the covalently bound substances; finally, through Break the cleavable groups on the surface of the magnetic beads, free the azide-labeled biomacromolecules, and achieve the purpose of enriching the azide-labeled biomacromolecules. Compared with the prior art, it has the advantages of simplified operation and improved efficiency. |