| abstract |
According to the invention, transformed cell lines, for example of Ustilago maydis, are used for detecting interactions between GPC-receptors (receptors with seven transmembrane sites, which interact with guanyl nucleotide-binding, regulatory proteins) or GPC-receptor-controlled signal transmission systems and test substances (ligands, modulators), or for searching for substances which are capable of interacting with receptors or signal transmission systems of this type. The transformed cell lines have a GPC-receptor-controlled signal transduction path with positive feedback and are transformed in such a way that they express a heterologous GPC- receptor. The transformed cell lines also contain a reporter gene, the expression of which can be detected using measuring techniques and which is controlled by a promotor. Said promoter can be induced by stimulating a GPC-receptor, and is endogenous. The reporter gene is endogenous or heterologous. |