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filingDate 1999-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3961325a28d546acf1a4b407747ccea4
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publicationDate 1999-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9941975-A1
titleOfInvention A method for the production of transgenic plants using apical shoot tips
abstract This application provides a method for transformation of cells of plant tissues and the regeneration of these cells and tissues into mature transgenic plants. The apical meristematic region of a plant seedling are isolated, then treated to slow the metabolic activity of the isolated tissue. The tissue is then exposed to a transforming agent such as a DNA plasmid or an Agrobacterium carrying a plasmid vector which contains a DNA encoding a gene of interest. After an appropriate incubation period, the treatment is reversed, allowing the metabolic activity of the apical meristematic tissues to return to normal. The method provides a larger population of receptive cells for exposure to the transforming agents, thereby providing higher probabilities of isolating transformed tissues which can be regenerated into transformed plants.
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