| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6d1c64916674986ae6826cb4eec69163 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8258
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-28 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate |
1998-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_238763762efeadb937e928283d5f710a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4dbf5e213d4a6eb52e0a69047b2da9fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6b43a8756c89f1fffb470855678f1d8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb475d42fceb6d021667e1244468a30b |
| publicationDate |
1999-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9918225-A1 |
| titleOfInvention |
Expression of cholera toxin b subunit in transgenic plants and efficacy thereof in oral vaccines |
| abstract |
A gene encoding the cholera toxin B subunit protein (CTB) is inserted into a plant expression vector. A preferred embodiment has the CTB gene fused to an endoplasmic reticulum (ER) retention signal (SEKDEL) adjacent to the mannopine synthase P2 promoter and has a bacterial luciferase reporter gene (lux F) linked to a P1 promoter. Potato leaf explants transformed by Agrobacterium tumefaciens carrying the vector and kanamycin-resistant plants are regenerated. The plant-derived CTB is antigenically indistinguishable from bacterial CTB, and oligomeric CTB molecules (Mr ∩ 50kDa) are the dominant molecular species isolated from transgenic potato leaf and tuber tissues. The maximum amount of CTB detected in auxin-induced transgenic potato leaf and tuber tissues is approximately 0.3 % of total soluble plant protein. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The expression of oligomeric CTB with immunological and biochemical properties identical to native CTB in edible plants permits inexpensive food plant-based oral vaccines for protection against cholera and other pathogens in endemic areas throughout the world. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7741536-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10689633-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9080180-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005096806-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1522585-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1685848-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102580118-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0172959-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9657302-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2169054-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2169054-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005033317-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0152886-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2005096806-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7041296-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102580118-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4769977-B2 |
| priorityDate |
1997-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |