| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f223fd81a50a52cd7d8328f186ea53d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8090a5ea39f9b1b1658845081b6952ab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_654a0affba937a5819915a45722a6da0 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-703
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1998-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a199552a047679181bd11ad10786983
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a430dd698c8afe45c250f4dc12efb12b |
| publicationDate |
1999-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9916910-A1 |
| titleOfInvention |
Method and kit for evaluation of hiv mutations |
| abstract |
A streamlined, hierarchical method for obtaining information about the allelic type of a sample of genetic material derived from an HIV-infected sample relies on the observation that 93-95 % of the known mutational variants of the reverse transcriptase and protease genes of HIV-1 can be determined by evaluating the positions of the A and T nucleotides within the sample. Thus, a substantial fraction of all mutational variations can be unequivocally identified by performing two initial sequencing reactions on the sample in which only ddA and ddT are used as chain terminators. For the small fraction of samples which are not identifiable based on the positions of these two bases, a second test is performed in which the sequence is determined in the 3'-direction for all four bases. This test will identify substantially all of the remaining samples. For those for which an ambiguity remains, however, a final test in which the sequence of the sample is determined in both the 3' and 5-direction for all four bases in performed. To perform the method, reagents suitable for performing the three tests within the hierarchy are suitably packaged as a kit containing two or more sub-kits. The first sub-kit contains reagents for performing A and T sequencing. The additional sub-kits contain reagents for performing a four-base sequence determination on one or both strands of the target DNA. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2395009-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2395008-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2395787-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2020450-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6623920-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2356703-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2356703-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7374877-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2395009-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2395008-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2395007-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9879053-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7741027-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9085807-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0220852-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6582920-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6946254-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7723040-B2 |
| priorityDate |
1997-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |