| abstract |
Methods for transforming plant, particularly commercial genotypes of cereals, are provided. The methods involve transformation of meristematic organogenic tissue, and include the use of defined plant growth media, including copper at a concentration from 0.1νM to about 50νM, the presence of at least one cytokinin and the absence or the presence of an auxin. The proliferation media may also include maltose at a concentration of up to 60 g/L. The methods disclosed provide more stable transgenic plants, and permit the transformation of varieties of cereals that are not amenable to tranformation by conventional approaches. |