abstract |
A chimeric Gα protein having yeast Gα (Gpa1p) amino acid sequences modified by a minimum of 3 amino acids positions within the C-terminal 10 amino acids by substitution by alternative amino acids, a transformed yeast cell comprising said chimeric Gα protein and a method of screening for a compound able to interact with a receptor comprising contacting a compound of interest with said yeast cell and observing the growth response of the cell or observing production of a reporter gene product. |