http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9910358-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_04e04aaf2e6e7215e508fdaaeb5af115 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 |
| filingDate | 1998-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41e2fafa7caa55256760736f845e0068 |
| publicationDate | 1999-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9910358-A2 |
| titleOfInvention | Method for producing nucleic acid polymers |
| abstract | The conventional synthesis of nucleic acid polymers from several oligonucleotides consists of several cycles of intermediate product synthesis, cleaning the intermediate product and the synthesis of a full length product (to a maximum length of approx. 1000 nucleotides). The novel method is carried out in a single reaction step and can result in nucleic acid polymers with more than 1000 nucleotides. According to the invention, two or more cross-linkable oligonucleotides are prepared. Said cross-linkable oligonucleotides can form a primary strand with continuous sequencing and after cross-linking. One or more non-cross-linkable oligonucleotides are also prepared. Each of the non-cross-linkable oligonucleotides has two adjacent areas, the first of which is complementary to the 3' end of a cross-linkable oligonucleotide and the second of which is complementary on the 5' end of another cross-linkable oligonucleotide. The cross-linkable oligonucleotides are hybridized with the complementary areas of the non-cross-linkable oligonucleotides and then cross-linked by enzymatic, chemical or photochemical means. The method is suitable for de novo-synthesis of long nucleic acid chains. |
| priorityDate | 1997-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.