http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9901548-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c0a6debc90a25a3144a1a2e21cea584 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b2a0586165c1d968dbdeeedc37324b27 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 1998-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1a5a2370b6491f2bee8d139dab89899 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3757fba5dab713ab0aab210615380440 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce0378d5db2c30846ee92591fb209052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89b526173d73f689b156341a50148526 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc3700575f779f54817138ce0e9a6a39 |
| publicationDate | 1999-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9901548-A3 |
| titleOfInvention | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
| abstract | The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag. Additionally, the invention provides a method for extracting a recombinant protein from within a recombinant Gram negative bacteria having a cell membrane, without lysing the bacteria, as well as a method for purifying an isolated recombinant human insulin, wherein the isolated recombinant human pro-insulin is subjected to sulfitolysis, Ni-chelation chromatography, renaturation, limited proteolysis and chromatography separation to provide purified, isolated, recombinant human insulin. |
| priorityDate | 1997-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 108.