| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d3d349d7cf2f15a6fb44540b7ffff40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0af1d874be0c3ea8bf1e271b2de61f1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_43a72884e25aaecffebe0942132d704f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca32fd2a44fd326ffd027a75a57bd13f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b538162930065b9a75827c25a0ba8cec
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f702b233abffd206ffcc727aefd55d8c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
| filingDate |
1998-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e43ab983dc0e3a7359beeffdfb4605c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2de23765b33ebf0839fc92385e0d044c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_efcce1c4aaa5f8473f3c8c7a8a8962b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9111fb49810cd125090874ac6ba2239 |
| publicationDate |
1998-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9859070-A1 |
| titleOfInvention |
METHOD FOR SELECTING cDNA FRAGMENTS |
| abstract |
This invention consists in a method for selecting cDNA fragments generated by differential display or RNA fingerprinting comprising the steps of: a) excising a differential band of the migration gel used for differential display or RNA fingerprinting and amplifying the corresponding cDNA fragments by PCR, b) submitting the PCR amplified cDNA fragments to gel electrophoresis in the presence of at least one polymer-coupled DNA binding agent and/or a polypeptide capable of DNA binding and mobility retardation, c) visualizing resolved cDNA fragments by means of a staining agent or radioactive labeling, d) excising the desired bands separated in step b) and directly amplifying the corresponding cDNA fragments by PCR, e) optionally purifying and analysing the amplified PCR products. |
| priorityDate |
1997-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |