http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9855636-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a3e7bb7512865a94b3991f488c20671 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b73e5863a2325f398bacdeaabdaefd46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b8641b7621c06c32b5d1aebcedf6bb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a8a033d7bf2fc58cc11ccb02f8170d8f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 |
| filingDate | 1998-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b252bcb913fb68c5aeb49a96ccc27688 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_641f609af5e34da3e1854cd218d57f91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5bc47fd5fc1d2142a9819d9f5f2c64e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_752e09ce339ba40ce0da93538901715e |
| publicationDate | 1998-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9855636-A1 |
| titleOfInvention | Recombinant construct for enhancement of gene expression in plants |
| abstract | The present inventions focuses on the super-expression of foreign genes in transgenic cells by combining within a single cDNA construct and respective RNA transcript, several trans- and cis-acting genetic elements of viral origin which will act in concert to trigger the following functional events: a) the primary chimeric continuous RNA transcript is produced by the transformed cells from plant-expressible promoter (35S promoter); b) RNA replicase produced by direct translation of the 5`-proximal gene of a single continuous primary transcript will synthesize secondary monocistronic (or dicistronic) mRNA as a result of the transcription from sgPr sequence. Expression of the 5`-proximal gene of these mRNAs will be enhanced by the αβ-leader. Translation of the 5`-distal gene of dicistronic mRNA will be promoted by internal ribosome entry site (IRES) sequence derived from crTMV tobamovirus mentioned above; c) it is probable that at least part of RNA transcripts originated from sgPr will include at their 3`-end the minus copy of RNA replicase gene and genomic promoter for plus-RNA synthesis. It can be expected that RNA replicase produced in transgenic cell will bind with the 3`-terminal sequence of this RNA (genomic promoter) producing upon transcription the RNA molecules carrying the plus-polarity replicase gene at the 5`-end. Translation of these mRNAs will result in production of additional replicase in transgenic plant. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1175144-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1175144-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02083867-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7348179-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02101006-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02101006-A2 |
| priorityDate | 1997-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.