| abstract |
A method of producing plants which exhibit an agronomically desirable trait comprises mutating or otherwise modifying in situ in a plant cell at least one gene which when modified is responsible for providing the said trait and regenerating from a cell exhibiting the said trait fertile morphologically normal whole plants, and is characterised in that a polynucleotide is introduced into the plant cell, the said polynucleotide comprising at least one region which is substantially complementary to at least one region in the gene, which gene region when mutated or otherwise modified provides for the agronomically desirable trait, the region in the said polynucleotide containing at least one base mismatch in comparison with the like region in the said gene, so that the region in the said gene is altered by the DNA repair/replication system of the cell to include the said mismatch. |