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filingDate 1997-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbf047416912b79cdff48a78aee6956c
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publicationDate 1998-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9849273-A1
titleOfInvention Raffinose synthetase gene, process for producing raffinose, and transformed plant
abstract A raffinose is produced by the action of a raffinose synthetase having the following properties on sucrose and galactinol: (1) activity and substrate specificity: raffinose is produced from sucrose and galactinol; (2) optimal pH: about 6-8; (3) optimal temp.: about 35-40 °C; (4) molecular weight: (i) molecular weight as measured by gel filtration chromatography: about 75-95 kDa; (ii) molecular weight as measured by polyacrylamide gel electrophoresis (native PAGE): about 90-100 kDa; and (iii) molecular weight as measured by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions: about 90-100 kDa; (5) inhibition: it is inhibited by iodoacetamide, N-ethylmaleimide, and myoinositol.
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