| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fdc007d8169755ab061e2fe540ccd8f4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ba045776bcd32d32e3100da8dfce4e28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2c658ed4639a11338cf0975a168ba120 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6887
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
1997-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c501ebf9a5d26ddc54d068a2e1f1f85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf6d06bae49e771e305c262697c6b273 |
| publicationDate |
1998-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9828436-A1 |
| titleOfInvention |
Screening assay for proteolysis inhibitors |
| abstract |
The invention concerns a method and assay (including a gel-based assay) for specifically identifying inhibitors of muscle protein degradation, comprising using (i) ubiquitin, (ii) a human ubiquitin-carrier E1 protein and (iii) a human ubiquitin-carrier E2 protein, which E2 protein is able to directly ubiquitinylate protein substrates and is expressed in skeletal and/or cardiac muscle (for example, E217k) and means for determining the extent of inhibition of ubiquitin transfer (for example by scintillation proximity assay (SPA)) to a target protein (for example biotinylated histone 2A). |
| priorityDate |
1996-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |