abstract |
A solid plating medium for the presumptive detection of Escherichia coli 0157:H7 which selectively promotes growth of Escherichia coli, inhibits growth of gram positive microorganims, inhibits growth of Proteus species, inhibits the growth of Escherichia coli other than Escherichia coli 0157:H7, wherein said plating medium includes agar, a carbohydrate medium which is not fermented by Escherichia coli 0157:H7 but is fermented by other microorganisms to include other strains of Escherichia coli and results in a first color when fermented, a chromogenic beta-galactosidase substrate which produces a second color when fermented by beta-galactosidase, and a pH indicator dye which changes said medium to a third color. |