Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcb146c68b67089013b4c7a614504254 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-182 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D413-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N31-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D271-12 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D413-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D271-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
filingDate |
1997-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78e121f399dfcb0b9b6fcdb9b45dcb4c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d5cc8044eaab5e049dda0fa316c18dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98ab943f02dabed03e22b8f527975969 |
publicationDate |
1997-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-9739141-A1 |
titleOfInvention |
Novel reagent, method, and kit for the quantitation of oxidation-reduction phenomena in proteins and peptides |
abstract |
A first embodiment of the method is for analyzing the amount of methionine sulfoxide in a protein sample and includes the steps of contacting a protein solution with methionine sulfoxide reductase in the presence of a reducing reagent bearing a covalently-linked reporter tag, whereby the reducing reagent is oxidized. The oxidized reducing reagent formed, which is proportion to the amount of methionine sulfoxide in the sample, is then quantified. A second embodiment of the method is for analyzing the amount of disulfide linkages in a polypeptide or protein sample. It proceeds in the same fashion as above, but in the absence of any enzyme. A novel fluorescently-labeled reducing agent, and kits to practice the method are also disclosed. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2019072565-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017123440-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104478823-A |
priorityDate |
1996-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |