http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9717438-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_512ebd45431df931808fb33dbb541ca8 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1086 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 1996-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_408000064d4701191497d8dbbc79cde8 |
publicationDate | 1997-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-9717438-A1 |
titleOfInvention | Method for expression cloning of gene encoding ligand-bound protein |
abstract | An efficient method for cloning a DNA which encodes a ligand-bound protein. The method comprises incubating on a plate a host into which a DNA inserted into an expression vector has been introduced; bringing a membrane into contact with the plate to thereby transfer the protein thus expressed from the plate onto the membrane; peeling off the membrane from the plate; adding a labeled ligand to the membrane so as to bind the ligand to the protein; detecting the label bound to the protein; and then isolating the DNA from a clone on the plate corresponding to the positive site on the membrane. |
priorityDate | 1995-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.