http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9710362-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efaece3f57d915a44fb0784b21caeab8 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 1996-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c64001e0fdbe7e37937b2d20c8b6ca4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80129f4c83baabb947a3c76de142ed10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd1e057b21a35d0175bb3649865602cf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14f90b20d40b8d65036b039aa6bc5370 |
publicationDate | 1997-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-9710362-A1 |
titleOfInvention | Methods of using nucleotide integrase for cleaving dna and attaching nucleic acids |
abstract | The present invention provides new methods, employing a nucleotide integrase, for cleaving double-stranded and single stranded DNA substrates at specific sites and for attaching nucleic acid molecules to the cleaved DNA substrates. One method uses a nucleotide integrase to cleave one strand of a double-stranded DNA and to concomitantly attach a nucleic acid molecule to the cleaved strand. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach a nucleic acid molecule to one strand of the DNA substrate. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach an RNA molecule to one strand of the substrate and for attaching a cDNA to the other strand of the substrate. Another method cleaves single stranded DNA with the concomitant insertion of a nucleic acid molecule at the cleavage point. The nucleotide integrase comprises an RNP particle which comprises a group II intron RNA bound to a group II intron encoded protein. The present invention also relates to purified and reconstituted RNP particles and reconstituted RNP that cleave DNA substrates. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2036984-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1774036-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10544422-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7592161-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-748237-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1774036-A2 |
priorityDate | 1995-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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