abstract |
The present invention consists in a method for producing a phage display vector which includes causing or allowing recombination between (i) a first vector which includes a sequence encoding a first polypeptide chain of a specific binding pair member; and (ii) a second phage vector which includes a sequence encoding cre-recombinase operatively linked to a control sequence which allows expression of the Cre recombinase and a sequence encoding a second polypeptide chain of a specific binding pair member; wherein the recombination event gives rise to a recombinant phage vector in which expression of the Cre recombinase is substantially inhibited. The present invention also consists in a phage vector which includes a nucleic acid sequence encoding a promoter sequence, a first recombination site downstream and adjacent to the promoter sequence and an open reading frame for cre-recombinase positioned downstream and adjacent to the recombination site such that the promoter drives expression of the Cre recombinase. |