| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d21cbff64022f95c237c0bd3ec8656ca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df273fdbe80daae7e5c81646e816944d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb32eedbbec73f50e658809f2a545ccf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be0f70351657b3ae7dfa41edaec12a19 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0075 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-245 |
| filingDate |
1996-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82f3c4ea0929ae8a8a09c6eabd249899
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34350212729a65c2912f0510434bd1b3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b409e15e8aed1d8d86a1fe54d8e84430 |
| publicationDate |
1997-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9708299-A1 |
| titleOfInvention |
Overproduction of neuronal nitric oxide synthase |
| abstract |
The present invention is directed to overproduction of nitric oxide synthase (NOS) in a prokaryote. More particularly, the invention involves overexpression of functional neuronal NOS in a protease-deficient strain of Escherichia coli, utilizing a pCW vector under the control of the tac promotor. The invention further involves co-expression of the protein with folding agonists, or chaperonins. The enzyme produced is complete with heme and flavins, and may be activated by incubation with tetrahydrobiopterin. It may be isolated as a predominantly high spin heme protein that demonstrates spectral properties which are identical to those of nNOS isolated from human kidney 293 cells. The methods disclosed are contemplated to be useful in expressing large amounts of other nitric oxide synthases, as well as other proteins that are difficult to produce correctly folded in prokaryotes. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9931220-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0037653-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1040184-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1040184-A4 |
| priorityDate |
1995-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |