| abstract |
A target nucleic acid sequence (12) may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer (A1, A2) that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridising with a 3'-terminal segment of the target and a DNA-type segment capable of hybridising with a segment adjacent to said 3'-terminal segment of the target, and a DNA- or RNA-type primer B1 capable of hybridising with said 3'-terminal segment of the target. A cyclic amplification is achieved that may be implemented isothermally on the basis of either a DNA or an RNA target even when the terminals are not defined. |