| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_544f50e6aa8884daf24470e3c2b4aec8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a2778f80d25e9cae24f296959e984414 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14222 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6862
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-35 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-015
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-35
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate |
1996-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1362938637739797ad2f4351dd151b0d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b40b9299ee4eb7c997626a9a12b6de6 |
| publicationDate |
1996-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9641005-A1 |
| titleOfInvention |
Methods for nucleic acid detection, sequencing, and cloning using exonuclease |
| abstract |
The present invention provides a method of detecting the presence of a nucleotide sequence within a double-stranded DNA in a sample comprising: (a) digesting the double-stranded DNA with an exonuclease which converts at least a portion of the double-stranded DNA to single-stranded DNA; (b) hybridizing the single-stranded DNA with i) a first nucleic acid probe adapted with a moiety which can be captured to a solid support, and ii) a second nucleic acid probe labeled with a detectable moiety which can hybridize with the single-stranded DNA adjacent the hybridized first nucleic acid probe; (c) ligating the hybridized first and second nucleic acid probes; (d) capturing the moiety on the first nucleic acid probe hybridized to the DNA to the solid support; (e) denaturing the ligated first and second nucleic acid probes from the hybridized single-stranded DNA; (f) removing uncaptured labeled probe; and, (g) detecting the presence of captured detectable moiety, the presence of captured detectable moiety indicating the presence of the nucleotide sequence within the double-stranded DNA in the sample. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10301686-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0118241-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016003197-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2456669-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0127320-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013079924-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02103013-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02103013-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2977465-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2456669-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7029882-B1 |
| priorityDate |
1995-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |