http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9640938-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14effe3850d2129f863b969dac4f1db8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1996-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0a6cc72b72d8b646c2b42f430d06750 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d992130f744208d1034bed528ccfaf1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62cf4171e5792d56b9cfcb4ac69893f8 |
| publicationDate | 1996-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9640938-A1 |
| titleOfInvention | Chondroitinase production in recombinant proteus vulgaris strains |
| abstract | Plasmids pLP2-1531, which encodes chondroitinases I and II, and pLP2-1521, which encodes chondroitinase I, are provided. These plasmids are used to transform wild-type E. coli and P. vulgaris cells. The transformed cells produce DNA encoding the production of chondroitinases I and II or chondroitinase I in the absence of natural exogenous chondroitinases I and II inducers. Additionally, the transformed P. vulgaris cells in the absence of exogenous chondroitinases I and II inducers, produce the respective enzymes in amounts in excess of those produced by wild-type P. vulgaris in the presence of exogenous chondroitinases I and II inducer(s). Starting plasmid pLP2-751 and intermediate plasmids pLP2-770, pLP2-1512, pLP2-1263, pLP2-1508, pLP2-1510, pLP2-1514, pLP2-1518, and pLP2-1525 are also provided. These intermediate plasmids are typically used in the preparation of pLP2-1531 and pLP2-1521. A BglII/EcoRI fragment of about 3970 bp derived from pLP2-1512, a EcoRI/SmaI fragment of about 2550 bp derived from pLP2-1514, and a BglII/SmaI(AvaI) fragment of about 6520 bp derived from pLP2-1518 are further disclosed. Also contemplated is the use of transformed P. vulgaris cells to produce chondroitinase I and/or II. The P. vulgaris cells above are cultured in a bacterial culture medium and in the absence of an exogenous chondroitinase I and II inducer. The cells are then harvested from the culture, and the chondroitinase I and/or II enzymes are recovered. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9796970-B1 |
| priorityDate | 1995-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.