abstract |
The present invention provides a method for increasing the levels of essential amino acids in seeds of plants, thereby enhancing the nutritional value of the seeds. The method comprises manipulating the metabolic pathway of the amino acid to provide an increased source of the target free amino acid and, concomitantly, over-expressing a preselected gene coding for the protein containing the target amino acid, such that there is accumulation of protein-bound target amino acid. A complementary protein sink is thus produced. The present invention is particularly useful in increasing levels of methionine, lysine and threonine in seeds. |