| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_05415f71448336cf43aa2df600727721
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b9bccc3cecfae2521e0649e6db2daf5d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec5b8c0fc1f827c1360a8f5ea5652225
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90f1eba8483f9e322b348c86899b05db
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a6ee60c69418bfb4101304d6dea5017a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C49-245 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate |
1996-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca4b2f2fa89ed874b29c1983f6017dd5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65b010824caa274f720143b044385c72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a79e4abaf2ea2084aaa473faad48c17
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b571f0fd73985b404bc92d8d7bd5d49a |
| publicationDate |
1997-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9637620-A3 |
| titleOfInvention |
Process for obtaining acyloins, pyruvate decarboxylases suitable therefor and their production and dna sequences of the pdc gene coding them |
| abstract |
The invention relates to a process for obtaining a pyruvate decarboxylase by isolation from a producer organism. The pyruvate decarboxylase is capable of forming (R)-(-)-phenylacetylcarbinole (I) in » 95 % enantiomer unit with a product ratio of (I) to 2-hydroxypropiophenone of » 95 %. In addition, the pyruvate decarboxylase has a specific activity with regard to phenylacetylcarbinole formation of > 1 U/mg. It is the aim of the invention to obtain a pyruvate decarboxylase with improved synthesis capacity concerning the formation of (R)-(-)-phenylacetylcarbinole. The process of the invention developed for this purpose is characterized in that use is made of a producing organism with a gene coding for pyruvate decarboxylase from Zymomonas mobilis, in the DNA sequence of which the tryptophane radical coding codon TGG is replaced at position 1174-1176 by a codon which codes for an amino acid radical with a reduced volume ratio. |
| priorityDate |
1995-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |