| abstract |
The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline α-amylase activity, alkaline α-amylase possessing both an amino acid sequence described in Sequence No. 3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in Sequence No. 4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline α-amylase activity. |