http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9634115-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f139884f8bc731c290911709f0249de0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5a5ae085853db5173d99daca7b6669 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a04326718c43dcbdb32230960ac8c380 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1996-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5acb5a18a5c15bc505e816d9eaeeaa07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7036d961d39d3f3f9cbdb0a6fbc346c3 |
| publicationDate | 1996-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9634115-A1 |
| titleOfInvention | Buffer and method for evaluating optimal nucleic acid target sequence amplification conditions and applications thereof |
| abstract | An application buffer, for facilitating nucleic acid denaturation while maintaining the structure of the enzymes used, and for significantly improving the performance of the polymerase chain reaction is described, as well as applications thereof, particularly in a method for amplifying nucleic acid target sequences using several temperatures and in a method for evaluating optimal conditions to be implemented in said amplification method. The buffer comprises, in addition to standard amplification buffer components, a mixture of at least one isodestabilising agent (AI) and at least one denaturing agent (AD), in concentrations capable of reducing the melting point (Tm) of the double-stranded nucleic acid target sequence, according to formula (4): Tm(°C)=81.5+0.41(% G+C)-ΔTmAI-675/N+16.6.logM-ΔTmAD, wherein ΔTmAI=f[(% G+C), (molarity of AI)] (formula 1), ΔTmAD=d(% denaturing agent) (formula 3), M: molarity of one or more monovalent cations present in the buffer, N: number of nucleotides, and d: efficiency coefficient of denaturing agent (AD), for reducing at least the denaturation temperature (Td) of said double-stranded nucleic acid target sequence, while allowing for the proper performance of the primer extension enzyme reaction. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9941410-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7387878-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2270233-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2005336287-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0123618-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7150980-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03042383-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0123618-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007028414-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1452593-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1452593-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6428986-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7384739-B2 |
| priorityDate | 1995-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.