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publicationDate 1996-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9630409-A1
titleOfInvention Method for immobilisation of proteins and polyelectrolytes on surfaces of solids
abstract Method of immobilization of proteins and polyelectrolytes on surfaces of solids in the form of films composed of molecular layers of proteins and polyelectrolytes arranged according to an eligible architecture characterised in that it includes the steps (a) the immobilisation of the protein or polyelectrolyte layer by the physical adsorption or a chemical bond, the step (b) the consecutive alternating adsorption of molecular layers of proteins and polyelectrolytes bearing opposite electrical charges, the step (c) the fixation of the resulting complex film by the chemical crosslinking and the step (d) the washing out of the uncrosslinked components.
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