| abstract |
The present invention relates to a putative protective antigen against a Mycoplasma, prepared by a method including a sample of a Mycoplasma; an antibody probe including at least one antibody against a Mycoplasma produced by a method including: providing a biological sample taken a short time after an immune animal has been challenged with a Mycoplasma or Mycoplasma extract taken from the infection site or an area of a lesion or an area close to the infection site or lesion; isolating cells from the biological sample; culturing cells in vitro in a suitable culture medium; and harvesting antibodies produced from said cells; probing the Mycoplasma sample with the antibody probe to detect at least one antigen; and isolating the antigen detected, also including diagnostic antigens, the preparation thereof, and their use in the formation of vaccine compositions, particularly vaccine compositions against Mycoplasma hyopneumoniae infections. |