abstract |
Disclosed are (1) a method of screening a DNA encoding a human cell growth inhibiting factor, by introducing a human DNA into a eukaryotic cell host under the control of an inducible promoter, and selecting DNA whose host cell does not grow under promoter-inducing conditions, but grows under non-promoter-inducing conditions, (2) new DNA encoding a human cell growth inhibiting factor selectable by this method, (3) a vector containing said DNA, (4) a transformant as transformed with said vector, (5) new human cell growth inhibiting factor, (6) a method for preparing said factor and a pharmaceutical composition containing said factor. According to the above screening method, a DNA encoding a human cell growth inhibiting factor can be selectively and conveniently obtained. The thus obtained DNA is useful as a probe for investigating aging, or as a reagent for diagnosing various aging-associated diseases. The human cell growth inhibiting factor can be useful as an anticancer agent, infection remedy and so on. |